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CAR T cell products were enriched for CAR T cells using the EasySep Human EGFR Positive Selection Kit (StemCell Technologies, 100-1131) according to the manufacturers instructions, and 150,000 CAR T cells per well (minimum of two wells per sample) were seeded in Seahorse XFe96 PDL cell culture microplates (Agilent, 103799-100)

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